Type of sample/quantity required: at least 0.6 ml of serum for both samples (pre and post stimulation with TSH): to obtain good quality serum it is essential to use tubes with separator gel and activator granules (these can be obtained from the laboratory by ordering them directly from the dedicated section of the online shop), wait for a clot to form, then centrifuge the sample for 10′ at 3000 RPM. The serum must then be separated as quickly as possible;

Storage/shipping methods: serum must be stored and shipped at refrigeration temperature;

Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued on the same day as the samples are received.

rhTSH STIMULATION TEST
1) Perform an initial sampling to measure the baseline T4;
2) Inoculate 75 µg/dog of recombinant human TSH (rhTSH) (Thyrogen) by IV
3) Perform a second sampling to measure the T4 post-stimulation, 6 hours after administering the drug.