EnglishHematology and Coagulation
Equipment currently used:
N. 3 SIEMENS ADVIA 2120 haematology systems;
N. 1 Beckman Coulter DXFLEX flow cytometer;
N. 1 SIEMENS BCS XP automatic coagulometer.
N. 10 optical microscopes for routine haematological/cytological tests
BLOOD GROUP DETERMINATION (DOG/CAT)
Type of sample/quantity required: whole blood in EDTA (must comply with blood to anticoagulant ratio)
Storage/shipping methods: blood samples must be stored and shipped at refrigeration temperature (avoiding direct contact with the freezer pack);
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued on the same day as the samples are received.
BONE MARROW IMMUNOPHENOTYPE (CYTOFLUORIMETRY)
THIS EXAMINATION MUST BE CARRIED OUT IN CONJUNCTION WITH A CYTOLOGICAL EXAMINATION OF THE BONE MARROW.
Type of sample/quantity required: bone marrow blood in EDTA (must comply with blood to anticoagulant ratio) + 3-4 unstained slides for cytological analysis: to enable correct interpretation of the flow cytometric results, the immunophenotype must be combined with a cytological analysis of the bone marrow.
Storage/shipping methods: samples must be stored and shipped at ambient temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 48 hours of reception.
HAEMATOLOGICAL IMMUNOPHENOTYPE (CYTOFLUORIMETRY)
THE EXAMINATION MUST BE CARRIED OUT IN CONJUNCTION WITH A COMPLETE HAEMOGRAM
Type of sample/quantity required: whole blood in EDTA (must comply with blood to anticoagulant ratio) + one unstained slide (it is important to smear the slide before the blood comes into contact with the EDTA); to enable the correct interpretation of flow cytometric results the immunophenotype must be carried out in conjunction with a complete haemogram. Upon special request, only the CD4 – CD8 ratio assessment can be performed;
Storage/shipping methods: samples must be stored and shipped at ambient temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 48 hours of reception.
Haematology: Coombs test
Sample type/quantity: Whole blood in EDTA (at least 0.5 ml).
Storage/shipping: Samples should be stored and shipped at refrigeration temperature.
Drop-off days: Daily from Monday to Saturday.
Days of execution/referral: Daily from Monday to Saturday.
Wait times: Reports are issued daily.
Haematology: minor/major crossmatch
Sample type/quantity: Whole blood in EDTA (at least 1 ml) from the donor and recipient.
Storage/shipping: Samples should be stored and shipped at refrigeration temperature.
Drop-off days: Daily from Monday to Saturday.
Days of execution/referral: Daily from Monday to Saturday.
Wait times: Reports are issued on the same day as drop-off.
HAEMOGRAM + RETICULOCYTE PROFILE + TEST FOR HAEMOPARASITES
Type of sample/quantity required: whole blood in EDTA (must comply with blood to anticoagulant ratio) + an unstained slide for microscopic analysis
Storage/shipping methods: blood samples must be stored and shipped at refrigeration temperature (avoiding direct contact with the freezer pack); slides must be placed in suitable slide holders;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued on the same day as the samples are received.
IMMUNOPHENOTYPING FOR ANTI-ERYTHROCYTE ANTIBODIES (CYTOFLUORIMETRY)
Type of sample/quantity required: whole blood in EDTA (must comply with blood to anticoagulant ratio)
Storage/shipping methods: samples must be stored and shipped at ambient temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 48 hours of reception.
IMMUNOPHENOTYPING FOR ANTI-PLATELET ANTIBODIES (CYTOFLUORIMETRY)
Type of sample/quantity required: whole blood in sodium citrate (must comply with blood to anticoagulant ratio)
Storage/shipping methods: samples must be stored and shipped at ambient temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 48 hours of reception.
COAGULATION PROFILE (PT, aPTT, Fibrinogen, FDP, D-dimers (dogs only), AT-III)
Type of sample/quantity required: 1 ml of plasma in 3.2% sodium citrate (it is essential to adhere to the 1:9 ratio with sodium citrate, centrifuge the sample at 3500 – 4000 RPM for 15’ then separate the plasma as soon as possible); individual coagulation profile tests can be requested;
Storage/shipping methods: plasma must be shipped as soon as possible at ambient temperature. The sample must not be subjected to thermal shock;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued on the same day as the samples are received.
SCREENING FOR COAGULATION FACTORS (FII, FV, FVII, FVIII, FIX, FX, FXI, and FXII)
Type of sample/quantity required: 1 ml of plasma in 3.2% sodium citrate (it is essential to adhere to the 1:9 ratio with sodium citrate, centrifuge the sample at 3500 – 4000 RPM for 15’ then separate the plasma as soon as possible); an assay of individual coagulation factors from the coagulation panel can be requested;
Storage/shipping methods: plasma must be shipped as soon as possible at refrigeration temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 30 working days of reception.
VON WILLEBRAND FACTOR (ELISA)
Type of sample/quantity required: 0.6 ml of plasma in 3.2% sodium citrate (it is essential to adhere to the 1:9 ratio with sodium citrate, centrifuge the sample at 3500 – 4000 RPM for 15’ then separate the plasma as soon as possible);
Storage/shipping methods: plasma must be shipped as soon as possible at refrigeration temperature;
Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 30 working days of reception.
