{"id":25859,"date":"2023-01-18T18:08:57","date_gmt":"2023-01-18T17:08:57","guid":{"rendered":"https:\/\/www.mylav.net\/nekategorizirano-sl\/nouveaux-bilans-pif-comment-bien-les-utiliser\/"},"modified":"2024-02-08T18:48:11","modified_gmt":"2024-02-08T17:48:11","slug":"novi-profili-fip-a-kako-ih-mozete-iskoristiti","status":"publish","type":"post","link":"https:\/\/www.mylav.net\/hr\/nekategorizirano-sl\/novi-profili-fip-a-kako-ih-mozete-iskoristiti\/","title":{"rendered":"NOVI PROFILI FIP-a: KAKO IH MO\u017dETE ISKORISTITI"},"content":{"rendered":"

Ove smo godine odlu\u010dili izraditi profile koji mogu biti od pomo\u0107i klini\u010darima pri dijagnosticiranju, kontroliranju i pra\u0107enju zaraznog peritonitisa ma\u010daka (FIP). U ovom blogu pogledajmo kako ih mozemo najbolje iskoristiti.<\/p>\n

Suhi oblik FIP-a: predla\u017eemo izradu opse\u017enog i sveobuhvatnog hematobiokemijskog profila kompletne krvne slike, kompletnu klini\u010dku biokemiju i elektroforezu.<\/p>\n

U osnovnu bazu podataka dodajemo dozu ma\u010djeg alfa-1 kiselog glikoproteina (fAGP) rije\u010d je o proteinu akutne faze koji je veoma koristan kada se sumnja na postojanje FIP-a \u2013 (pogledajte i najnoviju objavu na na\u0161em blogu: POVRATAK POZNATOG KLASIKA: MA\u010cJI AGP (ALFA-1 GLIKOPROTEIN)<\/a> \u2013 i dozu antitijela za ma\u010dji koronavirus tehnikom IFI (neizravne imunofluorescencije).<\/p>\n

Zapravo, problem za pacijente sa suhim oblikom FIP-a je nespecifi\u010dnost mnogih kroni\u010dnih upalnih klini\u010dko-patolo\u0161kih znakova: za kona\u010dnu potvrdu ovog oblika treba izravna identifikacija koronavirusa u parenhimatoznim o\u0161te\u0107enjima putem imunohistokemije i\/ili molekularne biologije (PCR). Rije\u010d je o invazivnim postupcima jer su potrebni uzorci biopsije i nisu uvijek jednostavni za izvo\u0111enje.
\nPrisutno\u0161\u0107u tipi\u010dnih hematobiokemijskih\/elektroforetskih promjena povezanih sa srednjom\/visokom serolo\u0161kom koncentracijom IFI-ja i visokom koncentracijom AGP-a znatno bi se podr\u017eala klini\u010dka sumnja na suhi oblik
\nFIP-a. Konkretno, kada je rije\u010d o analizi kompletne krvne slike radi FIP-a uklju\u010denjem pro\u0161irenog profila, mo\u017ee se omogu\u0107iti otkrivanje normokromne normocitne ili eventualno mikrocitne neregenerativne anemije povezane s neutrofilnom leukocitozom i\/ili limfopenijom.<\/p>\n

Biokemijskom slikom se mogu otkriti promjene jednog ili vi\u0161e organa me\u0111u kojima su osobito \u010deste promjene koje ukazuju na o\u0161te\u0107enje bubrega (pove\u0107anje kreatinina i uree) i jetre (povi\u0161ene vrijednosti ALT-a, ALP-a i bilirubina), ali prije svega hiperproteinemija, hipoalbuminemija i inverzija omjera albumina\/globulina.<\/p>\n

Ove posljednje promjene mogu se produbiti elektroforezom uklju\u010denom u profil kojim se obi\u010dno dokazuje porast za alfa2 i gamaglobuline tijekom trajanja FIP-a, kao i s odre\u0111ivanjem proteina fAGP koji je, kada postoji FIP, uobi\u010dajeno veoma visok (obi\u010dno > 1500 ug\/ml). <\/p>\n

\"Serumska
Serumska kapilarna elektroforeza ma\u010daka s FIP-om, hipoalbuminemijom i izra\u017eenom poliklonskom gamapatijom<\/figcaption><\/figure>\n

Mokri oblik FIP-a<\/strong>: u usporedbi sa suhim oblikom FIP-a mokrim se oblicima daje prednost provo\u0111enjem analize izlijeva tjelesnih \u0161upljina kojom se u mnogim slu\u010dajevima omogu\u0107ava potvr\u0111ivanje dijagnoze. Uz kompletnu hematobiokemijsku analizu i dozu proteina fAGP mo\u017ee se omogu\u0107iti otkrivanje gore opisanih tipi\u010dnih promjena istodobno provodimo citokemijsku analizu izljeva tjelesnih \u0161upljina i tra\u017eimo virus u uzorku putem PCR testa.<\/p>\n

Zapravo, izljevi (tjelesnih \u0161upljina) pacijenata s FIP-om imaju kemijska (povi\u0161ene koncentracije proteina, omjer albumina\/globulina \u010desto je < 0,8, povi\u0161eni LDH s visokim omjerom izme\u0111u LDH-a i ukupnog broja stanica u izljevu) i citolo\u0161ka (prisutnost asepti\u010dnog eksudata s niskom stani\u010dnom koncentracijom, proteinska pozadina i prevalencija nedegeneriranih neutrofilnih granulocita) obilje\u017eja kojima se upu\u0107uje na FIP.\nPozitivnim PCR testom teku\u0107ine potvr\u0111uje se 100 % dijagnoza FIP-a (visoka specifi\u010dnost), \u010dak i ako osjetljivost molekularne biologije nije apsolutna (osjetljivost oko 70 \u2013 80 %).\n \nPra\u0107enje FIP-a<\/strong>: kod ma\u010daka s potvr\u0111enom dijagnozom FIP-a predla\u017eemo profil za pra\u0107enje provedenih lije\u010denja primjenom osnovnog hematobiokemijskog profila i doze proteina fAGP. Klini\u010dko pra\u0107enje mora biti popra\u0107eno pobolj\u0161anjem tipi\u010dnih hematobiokemijskih promjena spe\u010dificnih za to oboljenje i smanjenjem proteina fAGP-a.<\/p>\n

Skloni\u0161ta za ma\u010dke, kolonije i farme<\/strong>: poznato je da koronavirus zaslu\u017ean za nastanak FIP-a potje\u010de od enteri\u010dkog koronavirusa ma\u010daka putem genetskih mutacija zbog kojih postaje sposoban zaraziti makrofage i prodrijeti u tkiva: \u0161to je ve\u0107i protok i replikacija enteri\u010dkog koronavirusa me\u0111u ma\u010dkama, ve\u0107a je vjerojatnost da se pojave slu\u010dajevi jasnog (razvijenog) FIP-a.<\/p>\n

U okru\u017eenjima s potencijalno visokom cirkulacijom ma\u010djeg koronavirusa na raspolaganju su nam dvije potencijalne metode za njegovo pra\u0107enje.<\/p>\n

Serologijom putem IFI-ja omogu\u0107ava nam se razumijevanje stupnja izlo\u017eenosti ma\u010daka u tom okru\u017eenju kada su u pitanju prethodne infekcije.<\/p>\n

Nadalje, tra\u017eenjem koronavirusa u stolici, s druge strane, omogu\u0107ava nam se razumijevanje koji mogu biti eliminacijski subjekti, \u010dak i ako treba imati na umu da uklanjanje virusa sa stolicom mo\u017ee biti nepravilno i remisivno \u010dak i za potpuno asimptomatske pacijente.<\/p>\n

Stoga je za pravilno sprje\u010davanje va\u017eno smanjiti, koliko je to mogu\u0107e, broj prisutnih ma\u010daka u okoli\u0161u i upotrijebiti serolo\u0161ke testove i\/ili testove PCR na stolici koji se ponavljaju<\/strong> tijekom vremena, provo\u0111enjem \u201enasumi\u010dnog uzorkovanja\u201c u svakoj grupi \u017eivotinja s promjenjivom u\u010destalosti ovisno o broju prisutnih ma\u010daka (\u010de\u0161\u0107e, npr.: jednom mjese\u010dno, ako ima mnogo ma\u010daka, s ve\u0107im vremenskim odmakom ako je broj ma\u010daka manji).<\/p>\n

\nSaverio Paltrinieri<\/strong>, vet. Europski specijalist veterinarske klini\u010dke patologije (EBVS) (dipl. ECVCP), Sveu\u010dili\u0161te u Milanu
\nWalter Bertazzolo<\/strong>, vet. Europski specijalist veterinarske klini\u010dke patologije (EBVS) (dipl. ECVCP), znanstveni direktor dru\u0161tva MYLAV\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Ove smo godine odlu\u010dili izraditi profile koji mogu biti od pomo\u0107i klini\u010darima pri dijagnosticiranju, kontroliranju […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-25859","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"\nNOVI PROFILI FIP-a: KAKO IH MO\u017dETE ISKORISTITI - MyLav<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.mylav.net\/hr\/nekategorizirano-sl\/novi-profili-fip-a-kako-ih-mozete-iskoristiti\/\" \/>\n<meta property=\"og:locale\" content=\"hr_HR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NOVI PROFILI FIP-a: KAKO IH MO\u017dETE ISKORISTITI - 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