Cytology and Histology

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Instrumentation currently in use:
N. 3 Automatic rotary microtomes (1 Shandon finesse ME+; 2 Bio-Optica 3005E)
N. 2 Automatic processors (Bio-Optica VTP 360)
N. 2 Embedding stations (Bio-Optica BEC150 and DP500)
N. 4 Histology fume hoods with vapour recirculation on filters (2 Bio-Optica Trimming Tech 180, Grosslab Junior, 1 Histoline Kim Activa)
N. 1 Block printer (TB586UV)
N. 1 Leica cryostat
N. 1 Immunostainer (Autostainer 480S)
N. 1 Automatic stainer (Bio-Optica HPC940)
N. 1 Mutomated coverslipper (CVR 909)

CYTOCHEMICAL EXAMINATION OF CAVITY EFFUSION

Type of sample/quantity required: it is essential to send a K3 EDTA tube containing the cavity fluid as sampled, the supernatant obtained by centrifuging the effusion at 3000 RPM for 10 minutes, collected in a tube with no anticoagulant, a slide obtained from a smear of the fluid as sampled and another smear of its sediment;

Storage/shipping method: When completely dry, the slides must be sent in suitable containers to avoid dust collecting on the smear. Test tubes must be shipped at refrigeration temperature (avoiding direct contact with the freezer pack).
IMPORTANT: any contact with formalin or its vapours must be strictly avoided (NEVER PLACE CYTOLOGICAL SAMPLES IN THE SAME BOX AS HISTOLOGICAL SPECIMENS FIXED IN FORMALIN).

Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 9 working days, subject to exceptions.

CYTOLOGICAL EXAMINATION OF HAEMOPOIETIC MARROW

Type of sample/quantity required: it is advisable to prepare multiple slides from a single sampling of the bone marrow, to increase the number of particles to be analysed. A tube of peripheral blood in K3 EDTA and a peripheral smear taken on the same day as the marrow sampling (or a blood count performed on the same day as the marrow sampling) must also be sent;

Storage/shipping method: Slides must be completely dry and sent in suitable containers to avoid dust collecting on the smear; K3 EDTA test tubes must be shipped at refrigeration temperature, avoiding direct contact with the freezer pack.
IMPORTANT: any contact with formalin or its vapours must be strictly avoided (NEVER PLACE CYTOLOGICAL SAMPLES IN THE SAME BOX AS HISTOLOGICAL SPECIMENS FIXED IN FORMALIN)

Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 8 working days, subject to exceptions.

GENERAL CYTOLOGICAL EXAMINATION

Type of sample/quantity required: Smears must be deposited on thoroughly clean, degreased new slides, whatever the type of material. Once this is done, the smeared material must be dried as quickly as possible in the air or using a heat source (e.g. radiator, hair-dryer, etc.);

Storage/shipping method: When completely dry, the slides must be sent in suitable containers to avoid dust collecting on the smear.
IMPORTANT: any contact with formalin or its vapours must be strictly avoided (NEVER PLACE CYTOLOGICAL SAMPLES IN THE SAME BOX AS HISTOLOGICAL SPECIMENS FIXED IN FORMALIN)

Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting times: reports are issued within 8 working days, subject to exceptions.

HISTOLOGICAL EXAMINATION

Type of sample/quantity required: excisional or incisional biopsies of pathological material for histological examination must be fixed in “10% buffered formalin solution for histology” (40% formaldehyde solution with phosphate buffer, to maintain a pH of 7 to 7.2). The use of other fixatives must be agreed with the laboratory.
Samples must be placed in wide-mouthed, unbreakable, airtight containers, suitable for holding ‘at least’ five times the volume of fixative as the volume of material to be examined. Formalin containers may be ordered directly from the laboratory.

Storage/shipping method: the speed of fixation, and therefore how soon preparation of the material can begin, depends on the size and thickness of the sample and the amount of fixative used.
Brief information about the sample (owner of animal, species, origin of material) must be written on the container. Accompanying sheets providing clinical/medical history data must be placed in envelopes and separated from containers carrying samples with fixation fluid.

Reception days: every day from Monday to Saturday;
Examination/reporting days: every day from Monday to Saturday;
Waiting time: the report will be issued within 9 working days from the date of acceptance if the samples arrive at the laboratory correctly fixed.